Melatonin in saliva ELISA

产品名称：Melatonin in saliva ELISA
产    地：Demeditec 
产品货号：DEKDSM96
产品规格：96 Tests
产品说明：
Special remarks:
The Direct Saliva Melatonin ELISA is a competitive immunoassay using a capture antibody (Ab) technique. The polyclonal Kennaway G280 anti-melatonin antibody has been coated onto the microtiter plate, provided in the kit. After the first 16-20 hours overnight incubation, melatonin present in the pre-treated saliva and controls as well as in the calibrators, compete with biotinylated melatonin during a second 3 hours incubation for the binding sites of this highly specific antibody.
After washing, the enzyme label, streptavidin conjugated to horseradish peroxidase (HRP) is added, which binds during a third 60 minutes incubation step to the melatonin-biotin antibody complexes captured on the coated wells. Unbound enzyme label is then removed by a second washing step and TMB substrate (tetramethylbenzidine) is added to the wells. In a fourth 30 minutes incubation step, a chromophore is formed in inverse proportion to the amount of melatonin present in the sample. The color turns from blue to yellow after the addition of an acidic stop solution and can be measured at 450 nm.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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