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Calcitonin ELISA

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产品名称: Calcitonin ELISA
产品型号: DEKAP0421
产品展商: 原装进口
产品文档: 无相关文档

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Calcitonin ELISA


Calcitonin ELISA  的详细介绍
Calcitonin ELISA

产品名称:Calcitonin ELISA
产    地:Demeditec 
产品货号:DEKAP0421
产品规格:96 Tests
产品说明:
Special remarks:
Biological activities
Calcitonin (CT) is a 32 amino acid peptide hormone secreted by the para-follicular C-cells of the thyroid gland under serum calcium control. After acute administration, this peptide acts as a potent hypocalcemic and hypophosphatemic hormone by increasing renal calcium clearance and reducing bone resorption. However, its precise physiological role in bone metabolism is not yet fully understood. Various forms of CT may be detected in blood samples, including a CT monomer, an oxidized monomer, a dimer, higher molecular weight forms, and possibly precursor of CT. The concentrations of these peptides vary with clinical status, renal function and tissular origin of CT (normal or ectopic production). Medullar thyroid carcinoma (MTC) is a malignant tumor, developed from the C-cells, secreting calcitonin in large excess. This disease occurs either as a sporadic (80%) or a familial (20%) form, which is transmitted as an autosomal dominant gene or as a component of multiple endocrine neoplasia (IIb). Moderate hypercalcitoninemia is also observed in pregnancy, pernicious anaemia, renal failure and during the neonatal period. Preferably, monomer form of CT is detected in this assay.
Clinical Application
The measurement of CT is used for:
· Diagnosis of medullary thyroid carcinoma (MTC),
· Follow up of malignant tumors, to check the success of surgery and to monitor for recurrence,
· Diagnosis of the preclinical cases of the familial forms of MTC (MEN II or Sipple syndrome) by the use of stimulation tests (calcium or pentagastrin),
·Study of the pathophysiology of the calcium-phosphate and bone metabolism.
PRINCIPLES OF THE METHOD
The Demeditec CT-U.S.-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human CT – MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the Calcitonin concentration. A calibration curve is plotted and Calcitonin concentration in samples is determined by interpolation from the calibration curve.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位,公司提供的产品涵盖了二十多个国家近五十万种产品,而且产品的数量与信息在不断的增长和更新,公司提供的产品能够使生命科学工作者加快对生物化学,分子生物学,细胞生物学以及蛋白质组学研究的认知,以及分子诊断和临床医学领域的应用。

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