Lightning-Link Biotin (Type A - 3 reactions up to 20ug)

产品名称：Lightning-Link Biotin (Type A - 3 reactions up to 20ug)
产    地：Innova Biosciences
产品货号：704-0030
产品规格：Type A - 3 reactions up to 20ug
产品说明：
Lightning-Link kits
Lightning-Link? is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link? process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.
The Lightning-Link? range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link? conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.
Biotin Label
The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA.  The biotin in the kit has an extended linker to facilitate molecular interactions.
Lightning-Link? Biotin has been optimised for two separate applications. Type A is intended for assays in which a streptavidin-labeled detection reagent will be used, whilst Type B is optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
Manufacturing
The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.
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