4α-Phorbol 12,13-Didecanoate **

产品名称：4α-Phorbol 12,13-Didecanoate, >99%
产品货号：LC  P-2170
产品规格：1 MG
Negative control for studies with Phorbol 12,13-Didecanoate, formerly our Cat. No. P-1925, for example, see Trewyn, R.W. and Gatz, H.B.  "Altered growth properties of normal human cells induced by phorbol 12,13-didecanoate."  In Vitro. 20:  409-15 (1984).
Please request Technical Note #13 for additional information.
4α-PDD Activation of TRPV4 Channels  Long thought to be a biologically inactive or extremely weak phorbol ester analog (i.e., an ED50 >25 µM for binding to protein kinase C), 4α-PDD has now been shown to be a reasonably potent activator of two TRPV4 channels, namely human VRL-2 and murine TRP12 channels [Watanabe, H., et al.  "Activation of TRPV4 channels (hVRL-2/mTRP12) by phorbol derivatives."  J. Biol. Chem. 277:  13569-13577 (2002)].  The ED 50 of 4α-PDD for activation of the TRP12 channel was ~400 nM, and for increasing internal calcium levels in 1321N1 astrocytoma cells expressing human VRL-2, the ED50 of 4α-PDD was ~185 nM.  This work extends earlier results showing non-phorbol-ester-like effects of 4α-PDD [Reeve, H., et al.  "Enhancement of Ca2+ channel currents in human neuroblastoma (SH-SY5Y) cells by phorbol esters with and without activation of protein kinase C."  Pflugers Arch. Eur. J. Physiol. 429:  729-737 (1995)] and 4α-phorbol 12,13-dibutyrate (4 -PDBu) [Doerner, D., et al.  "Protein kinase C-dependent and -independent effects of phorbol esters on hippocampal calcium channel current."  J. Neurosci. 10:  1699-1706 (1990)] on calcium currents.
Storage:  Store at or below -20 ºC.  Solubility:  Soluble in DMSO or ethanol.  Disposal:  A

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