Nuclear RED (DRAQ5) **

产品名称：Nuclear RED (DRAQ5)
产品货号：eBioscience 65-0880-96
产品规格：200 uL 
DRAQ5™
RUO: For Research Use Only. Not for use in diagnostic procedures.
SKU# 65-0880
Cat. No. Size
65-0880-92  50 uL 
65-0880-96  200 uL 
Description: DRAQ5™ is an anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells. In flow cytometry, this dye can be used to distinguish nucleated and non-nucleated cells. DRAQ5™ can also be used to report nuclear DNA content for ploidy and cell cycle analysis because it binds DNA stoichiometrically. In fluorescent microscopy, it can be used as a nuclear counterstain.
DRAQ5™ can be excited by wavelengths from 488 to 647 nm. For microscopy/imaging, 633 or 647 nm sources for excitation are recommended. For flow cytometry, with excitation at 488 nm, this dye can be detected with filter sets designed for PerCP-eFluor® 710 or PerCP-Cy5.5 (685LP dichroic mirror and 710/50 band pass); with excitation at 633 nm, it can be detected with filter sets designed for APC (660/20 band pass). For cell cycle/DNA analysis applications, a higher wavelength filter is recommended, such as a 710LP or a filter set designed for PE-Cy7 (735LP dichroic mirror and 780/60 band pass), to optimize the CV for the G1 and G2/M peaks. Please make sure that your instrument is capable of detecting this dye.
Because of its broad excitation and emission properties, DRAQ5™ is not recommended in combination with other far-red fluorochromes excited by the 488 or 633 nm laser lines, including PE-Cy7, PerCP-eFluor® 710, PerCP-Cy5.5, APC, Alexa Fluor® 647, Alexa Fluor® 700, APC-eFluor® 780 and equivalents (APC-Alexa Fluor® 750 and APC-Cy7).

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