Fixable Viability Dye eFluor450, **

产品名称：Fixable Viability Dye eFluor450, 5x100 tests
产品货号：eBioscience 65-0863-18
产品规格：500 tests
Fixable Viability Dye eFluor® 450
RUO: For Research Use Only. Not for use in diagnostic procedures.
SKU# 65-0863
Cat. No. Size
65-0863-14  100 tests 
65-0863-18  500 tests 
Description: Fixable Viability Dye eFluor® 450 is a viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. Fixable Viability Dyes may be used to label cells from all species.
Fixable Viability Dye eFluor® 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor® 450 or Pacific Blue®). Please make sure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
Fixable Viability Dye eFluor® 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture. Store at -80°C with dessicant. It may be freeze-thawed up to 20 times. Allow vial to equilibrate to room temperature before opening.

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!