Indo-1, AM UltraPure Grade 1 pack

产品名称：Indo-1, AM *UltraPure Grade* 1 pack (20 x 50 ug vials)
产品货号：eBioscience 65-0857-78
产品规格：1 mg
Indo-1 AM Calcium Sensor Dye (UltraPure Grade)
RUO: For Research Use Only. Not for use in diagnostic procedures.
SKU# 65-0857
Cat. No. Size
65-0857-78  20 x 50 ug 
Description: Indo-1 AM Calcium Sensor Dye is a membrane-permeable dye used for determining changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye. Unbound Indo-1 has a peak emission at 485 nm. Upon binding calcium, the peak emission shifts down to 410 nm.
In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths. To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 485nm (525 nm is a good option) while bound Indo-1 fluorescence should be collected using a filter below 400 nm. Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eFluor™605NC or eFluor™ 650NC is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1.
Molecular Weight: 1009.91
Peak Excitation: 346 nm
Peak Emission: 475 nm
Indo-1 AM should be reconstituted in high-quality, freshly opened DMSO. Recommended concentration: 1 mM.
Once reconstituted, it should be protected from light and stored at -20°C; avoid freeze-thaws.

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