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Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase,

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产品名称: Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase,
产品型号: Sigma P6782 10mg
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Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase, Horseradish 现货


Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase,  的详细介绍
Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase, Horseradish 现货

辣根过氧化物酶

过氧化物酶，酶学分类号为EC 1.11.1.7。该酶催化

　　Donor+ H2O2--→Oxidized donor+2 H2O。

　　过氧化物酶，通常来源于辣根（因此称辣根过氧化物酶），是临床检验试剂中的常用酶。该产品不但广泛用于多个生化检测项目，也广泛运用于**类（ELISA）试剂盒。过氧化物酶作为多个试剂盒显色体系的关键成分，对试剂盒的质量有重要影响。

　　辣根过氧化物酶(Horseradish Peroxidase, HRP)比活性高，稳定，分子量小，纯酶容易制备，所以*常用。HRP广泛分布于植物界，辣根中含量高，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18％。HRP由多个同功酶组成，分子量为40,000,等电点为PH3～9,酶催化的*适PH因供氢体不同而稍有差异，但多在PH5左右。酶溶于水和58％以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白*大吸收光谱分别为403nm和275nm，一般以OD403nm ／OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。高纯度的酶RZ值应在3.0左右(*高可达3.4)。RZ值越小，非酶蛋白就越多。

Sigma相关产品资料：

Sigma  P6782  Peroxidase from horseradish

Type VI-A, essentially salt-free, lyophilized powder, ~1000 units/mg solid (using ABTS), 250-330 units/mg solid (using pyrogallol)

Synonym:

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

CAS Number:

9003-99-0

Enzyme Commission (EC) Number:

1.11.1.7  ( BRENDA  | IUBMB  )

EC Number:

232-668-6

MDL number:

MFCD00071339

 

Description

Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

 

This product is assayed using ABTS for easy comparison to other suppliers' unit: approx. 1,000 units per mg solid

Linkage

Similar to P8375

Other Notes

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Packaging

Packaged in mg solid

Unit Definition

One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0

 

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

 

性质

type

Type VI-A

form

essentially salt-free, lyophilized powder

EQP level

Premium

storage temp.

2-8°C

 

**性

Personal Protective Equipment

Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

WGK Germany

3

 

品名

产地

货号

规格

单价

备注

HRP辣根过氧化物酶

Sigma 

P6782

10mg

询价

现货

HRP辣根过氧化物酶

Sigma 

P6782

25mg

询价

现货

HRP辣根过氧化物酶

Sigma 

P6782

50mg

询价

现货

HRP辣根过氧化物酶

Sigma 

P6782

100mg

询价

现货

 

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