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反转录酶

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产品名称: 反转录酶
产品型号: invitrogen 18080-044
产品展商: Invitrogen
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简单介绍

SuperScript III Reverse Transcriptase 反转录酶III 逆转录酶 美国invitrogen公司原装进口


反转录酶  的详细介绍

SuperScript III Reverse Transcriptase
反转录酶III     逆转录酶

Description

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. The structural modifications provide:
  • Half life of 220 minutes at 50°C for the highest cDNA yields (Figure 1)
  • Reduced RNase H activity for more full-length cDNA
  • Full activity at 50°C for increased specificity with GSP
  • Ability to increase RT units without inhibiting subsequent PCR
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