小鼠超灵敏胰岛素检测试剂盒|超灵敏胰岛素检测试剂盒|胰岛素检测试剂盒|超敏胰岛素检测试剂盒
Ultra Sensitive Mouse Insulin ELISA Kit
窗体顶端
Crystal Chem Inc
Catalog No: 90080
Sample Size: 5 µL
Sample Types: Serum, plasma, cell culture, or fluid
Tests: 96 wells (8 wells x 12 modules)
Reagents: In liquid form (except standard)
Assay Range: Low Range Assay: 0.1 - 6.4 ng/mL
Wide Range Assay: 0.1 - 12.8 ng/mL*
High Range Assay: 1 - 64 ng/mL
* Intended for screening purposes
Assay Time: Same day procedure
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage: 2-8°C
Regulatory: Research Use Only
Distribution: Worldwide
Product Details
Recovery: When mouse insulin was spiked to mouse serum, the recovery of mouse insulin was 100% ± 15%
Specificity: Substance Reactivity
Mouse Insulin 100%
Rat Insulin 100%*
Hamster Insulin Detected*
Rat C-peptide Not detected
Rat pancreatic polypeptide Not detected
Porcine glucagon Not detected
Human Insulin like growth factor-I Not detected
Human Insulin like growth factor-II Not detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit
Typical Standard Curve:

Highlights: Small Sample Volume: Only 5 µl
High Sensitivity:0.1 ng/ml using 5 µl sample and 0.005 ng/ml using 100µl sample
Low and High Insulin Assay: Both using the same kit
Precision: Intra-assay and inter-assay precision CV ≤ 10.0%
Dynamic Range: 0.1-64.0 ng/ml using 5 µl sample
Effect of Hemolysis: Almost no effect of hemolysis tested up to 20mg/ml.
90060 + 90330 | Hamster Insulin ELISA Kit |
90060 + 90180 | Rabbit Insulin ELISA Kit |
90060 + 90150 | Dog Insulin ELISA Kit |
90010 | Rat Insulin ELISA Kit |
90040 | Rat Leptin ELISA Kit |
90030 | Mouse Leptin ELISA Kit |
90060 | Ultra Sensitive Rat Insulin ELISA Kit |
90080 | Ultra Sensitive Mouse Insulin ELISA Kit |
ALPCO
Mouse Ultrasensitive Insulin ELISA
窗体底端
Catalog Number: | 80-INSMSU-E01 |
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Description: | Mouse Ultrasensitive Insulin ELISA |
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Species Types: | Mouse |
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Sample Types: | Plasma, Serum |
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Sample Sizes: | 5 µL, 25 µL |
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Available Sizes: | 96 Wells |
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Range: | 0.025 - 6.9 ng/mL |
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Sensitivity: | 0.115 ng/mL (5 µL), 0.019 ng/mL (25 µL) |
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Incubation: | 2 hour(s) 30 minute(s) |
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Protocol: | Mouse Ultrasensitive Insulin ELISA |
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Material Safety Data Sheet: | Mouse Ultrasensitive Insulin ELISA |
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Regulatory Status: | Research Use Only. Not for Use in Diagnostic Procedures. |
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Product Distribution: | Available Worldwide |
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Related Products: | Mouse Insulin ELISA 80-INSMS-E01 Mouse Insulin ELISA Jumbo 80-INSMS-E10 Mouse High Range Insulin ELISA Jumbo 80-INSMSH-E10 Mouse High Range Insulin ELISA 80-INSMSH-E01 Mouse Ultrasensitive Insulin ELISA Jumbo 80-INSMSU-E10 |
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Literature References: | More Insulin ELISA Options |
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· Summary:
· Performance Characteristics
· Publications
· Related Information
The ALPCO Mouse Ultrasensitive Insulin ELISA kit quantifies the concentration of the insulin protein products of the mouse I and mouse II proinsulin genes from as little as 5µl of serum or plasma sample. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Mouse Ultrasensitive Insulin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time. Additional information regarding the Mouse Ultrasensitive Insulin ELISA kit can be accessed by clicking on the “Protocol” link or “Support” tab above.
The ALPCO Mouse Ultrasensitive Insulin ELISA is provided with seven standards designed to meet the needs of either very small sample size, or very low insulin concentrations. For small samples, such as tail snip samples, use a sample size of 5µl and the top five standards, 0.18-6.9 ng/ml. For hypoinsulinemic samples from a type 1 diabetes model or streptozotocin treatment, use a 25µl sample size and the bottom five standards, 0.025-1.25 ng/ml.
When more than 280 samples need to be assayed in duplicate, the ALPCO Mouse Ultrasensitive Insulin ELISA Jumbo/10-plate format is the economical option. Click on “Mouse Ultrasensitive Insulin ELISA Jumbo 80-INSMSU-E10” above and learn more about reducing the cost associated with quantifying insulin in a large number of samples.
If additional Zero Standard is required, click on the “Zero Standard” link in the “Related Product” section to order this product.
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Mercodia 10-1247-01 Mouse Insulin ELISA 小鼠胰岛素ELISA试剂盒
Mercodia—糖尿病与心血管研究的先驱者
瑞典Mercodia是****的生产高品质诊断**试剂的开发商,制造商和分销商,专注于将酶联**试剂盒用于心血管**,糖尿病和肥胖的临床以及研究,主要提供人和哺乳动物的检测试剂盒.
Mercodia成立于1991年,总部在瑞典乌普萨拉,生产的产品超过百分之九十出口到100多个国家。在美国设有子公司,位于温斯顿塞勒姆,北卡罗来纳,直接服务于北美客户。
现货供应--瑞典Mercodia公司大鼠/小鼠胰岛素试剂盒
瑞典Mercodia公司的大鼠/小鼠胰岛素试剂盒在国内市场取得了优良的销售记录,针对长期客户和急用客户,我公司长期备有大量现货。
产品名称: 大鼠胰岛素ELISA试剂盒小鼠胰岛素ELISA试剂盒
英文名称: Rat Insulin ELISA Kit |Mouse Insulin ELISA
产品货号: 10-1250-01|10-1247-01
产品规格: 96 wells |96 wells
产品产地: 瑞典
品牌商标:Mercodia
价 格: 3416元
保存与运输: 2-8℃
现货状态: 现货供应
产品描述:
胰岛素是胰岛β细胞分泌的一种蛋白质**,分子量为6KD,属于结构相关调节蛋白家族,这一家族其他的蛋白还包括类胰岛素生长因子、骨松弛素。胰岛首先合成分子量为12KD的胰岛素原,其后胰岛素原在细胞内经过加工处理,形成分子量为9KD的胰岛素原并被包裹成颗粒。这些颗粒再进行裂解,便形成了胰岛素和C肽,在胰岛受到刺激后被分泌到血液中。
胰岛素是调节糖代谢的重要**,也是体内**能降低血糖的**。它的合成速度受血浆葡萄糖浓度的影响。它在体内其他**的配合下,调节糖、蛋白质、脂肪的合成与分解代谢。其作用包括如下:促进糖原合成,抑制糖原分解,抑制葡萄糖产生,促进糖的利用。促进脂肪、蛋白质合成,抑制脂肪、蛋白质分解。促进DNA、RNA、ATP的合成。
胰岛素浓度升高见于以下**:胰岛β细胞瘤或胰岛β细胞增生、II型糖尿病或某些胰岛素初期患者、胰岛素自身**综合征、继发性糖尿病、胰岛素结构异常、胰岛素受体异常、肝硬化、肥胖、妊娠、口服避孕药等。
胰岛素浓度降低见于以下**:I型糖尿病、胰腺炎或胰腺炎并发胰岛功能损伤。
人糖尿病ELISA试剂盒
产品编号产品名称规格价格产地
10-1193-01 Adiponectin ELISA 96 tests 8285 Mercodia
10-1199-01 Leptin ELISA,瘦素 96 tests 5980 Mercodia
10-1113-01 Insulin ELISA,胰岛素 96 tests 3610 Mercodia
10-1113-10 Insulin ELISA,胰岛素 10×96 tests 32415 Mercodia
10-1132-01 Ultrasensitive Insulin ELISA,超敏胰岛素 96 tests 4040 Mercodia
10-1128-01 Iso-Insulin ELISA,同功胰岛素 96 tests 5810 Mercodia
10-1136-01 C-peptide ELISA,C-肽 96 tests 4955 Mercodia
10-1141-01 Ultrasensitive C-peptide ELISA,超敏C-肽 96 tests 4955 Mercodia
10-1118-01 Proinsulin ELISA,胰岛素原 96 tests 5980 Mercodia
10-1222-01 Insulin Pre-Transplantation ELISA,胰岛素移植前 96 tests 4850 Mercodia
动物糖尿病ELISA试剂盒
产品编号产品名称规格价格产地
10-1232-01 Rat/Mouse Proinsulin ELISA,大鼠/小鼠胰岛素原 96 tests 8210 Mercodia
10-1250-01 Rat Insulin ELISA,大鼠胰岛素 96 tests 3685 Mercodia
10-1250-10 Rat Insulin ELISA,大鼠胰岛素 10×96 tests 33100 Mercodia
10-1251-01 Ultrasensitive Rat Insulin ELISA,超敏大鼠胰岛素 96 tests 4110 Mercodia
10-1145-01 High Range Rat Insulin ELISA,宽线性大鼠胰岛素 96 tests 3685 Mercodia
10-1145-10 High Range Rat Insulin ELISA,宽线性大鼠胰岛素 10×96 tests 33100 Mercodia
10-1172-01 Rat C-peptide ELISA,大鼠C-肽 96 tests 8285 Mercodia
10-1247-01 Mouse Insulin ELISA,小鼠胰岛素 96 tests 4510 Mercodia
10-1247-10 Mouse Insulin ELISA,小鼠胰岛素 10×96 tests 40540 Mercodia
10-1249-01 Ultrasensitive Mouse Insulin ELISA,超敏小鼠胰岛素 96 tests 4900 Mercodia
10-1249-10 Ultrasensitive Mouse Insulin ELISA,超敏小鼠胰岛素 10×96tests 44060 Mercodia
10-1200-01 Porcine Insulin ELISA,猪胰岛素 96 tests 5780 Mercodia
10-1201-01 Bovine Insulin ELISA,牛胰岛素 96 tests 5780 Mercodia
10-1202-01 Ovine (Sheep) Insulin ELISA,羊胰岛素 96 tests 5780 Mercodia
10-1223-01 High Range Porcine Insulin ELISA,宽线性猪胰岛素 96 tests 6090 Mercodia
10-1203-01 Canine Insulin ELISA,,狗胰岛素 96 tests 5780 Mercodia
10-1204-01 Equine Insulin ELISA,马胰岛素 96 tests 5780 Mercodia
10-1256-01 Porcine C-peptide ELISA,猪C-肽 96 tests 9420 Mercodia
10-1251-10 Ultrasensitive Rat Insulin ELISA,超敏大鼠胰岛素 10×96 tests 36895 Mercodia
10-1248-01 Insulin FIA Rat/Mouse,大鼠/小鼠荧光法胰岛素 10×96 tests 87710 Mercodia
10-1205-01 Equine Insulin ELISA,,马胰岛素 96 tests 5780 Mercodia
10-1233-01 Feline insulin ELISA,猫胰岛素 96 tests 5780 Mercodia
10-1137-10 Ultrasensitive Rat Insulin ELISA,超敏大鼠胰岛素 10×96 tests 31824 Mercodia
人心血管ELISA试剂盒
产品编号产品名称规格价格产地
10-1106-01 Apo(a) ELISA,载脂蛋白a 96 tests 5490 Mercodia
10-1143-01 Oxidized LDL ELISA,氧化低密度脂蛋白 96 tests 10235 Mercodia
10-1158-01 Oxidized LDL Competitive ELISA,氧化低密度脂蛋白竞争法 96 tests 10235 Mercodia
10-1176-01 MPO ELISA,髓过氧化物酶 96 tests 9800 Mercodia
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Millipore(密理博) EZRMI-13K
Rat/Mouse Insulin ELISA
Qty/Pk: 96-well strip plate
Background Information: Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
Key Applications: ELISA
Application Notes: 3 hour assay, room temperature 10 μL sample size
Species Reactivity: Rat Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Linearity of Dilution: 79–133%
Sensitivity: 0.2 ng/mL
UniProt Number: P01322 P01325
Precision, %:
Inter-assay: 6.0–17.9
Intra-assay: 0.9–8.4
Accuracy: 83–102%
Detection Methods:
Fluorescent
Colorimetric
Product Name: Rat/Mouse Insulin ELISA
Standard Curve Range: 0.2–10 ng/mL
Analytes Available: Insulin
操作步骤:(详细见英文说明书,可应客户需求提供中文说明书)
糖尿病研究相关试剂盒(Millipore):
Human Insulin ELISA,货号:EZHI-14K,规格:96-well strip plate
Human Intact Proinsulin ELISA, 货号:EZHIPI-17K,规格:96-well strip plate
Human Total Proinsulin ELISA,货号:EZHPI-15K,规格:96-well strip plate
Glucagon Like Peptide-1 (Active) ELISA,货号:EGLP-35K,规格:96-well plate
Description: 背景介绍:
∷胰岛素是由胰岛β细胞受内源性或外源性物质如葡萄糖、乳糖、核糖、精氨酸、胰高血糖素等的刺激而分泌的一种蛋白质**,其分子量为5808 Da。胰岛素是机体内**降低血糖的**,同时促进糖原、脂肪、蛋白质合成。胰岛素的作用还包括:增强糖原的合成,增强脂肪酸的合成,增强脂肪酸的酯化作用,降低蛋白降解,降低脂肪降解,降低葡萄糖的合成,增加氨基酸的摄入,增加钾元素的摄入,动脉肌肉张力调控。
∷胰岛素升高与**:胰岛β细胞瘤或胰岛β细胞增生、II型糖尿病或某些胰岛素初期患者、胰岛素自身**综合征、继发性糖尿病、胰岛素结构异常、胰岛素受体异常、肝硬化、肥胖、妊娠、口服避孕药等。
∷胰岛素降低与**:I型糖尿病、胰腺炎或胰腺炎并发胰岛功能损伤。
检测样品:大小鼠血清,血浆样品。其他生物样品客户可自行验证实用性。
重要参数:
∷灵敏度:*低检测0.1ng/ml(10 ul样品)
∷线性检测范围:0.1 ~10 ng/mL(10 ul样品)
∷**度:83–102%
检测原理:
Rat/Mouse Insulin ELISA采用双抗体夹心ELISA法,1)96孔的微孔板预涂上小鼠单克隆抗体用来捕获样品中的胰岛素分子。生物素标记的多克隆抗体结合到捕获的胰岛素上。2)洗去未捕获的胰岛素。3)加入链霉亲和素标记的辣根过氧化物酶(HRP),选择性地与生物素标记的**复合物结合。4)洗去未结合的酶。5)加入3,3′,5,5′ - 四甲基联苯胺(TMB)作为反应底物,通过测定HRP活性来检测被固定的抗体-酶复合物。加入终止液酸化后,在450nm测定酶活性。吸收峰的增加与未知样品中捕获的胰岛素数量成正比,可通过绘制标准曲线求出样品中胰岛素浓度。
试剂盒组分与保存温度(产品效期见盒子外包装):
编号组分名称规格保存温度
A预包被好的酶标板 (96孔),可拆卸 1 plate2~8℃
B封板膜1 sheet2~8℃
C10×HRP Wash Buffer(浓缩液)2*50 mL 2~8℃
DRat/Mouse 胰岛素标准品(0.2, 0.5, 1, 2, 5,10 ng/mL)0.25 mL/vial 2~8℃
ERat/Mouse 胰岛素对照1和20.25 mL/vial 2~8℃
F基底缓冲液0.5 mL 2~8℃
G检测稀释缓冲液20 mL 2~8℃
HRat/Mouse 胰岛素检测抗体10 mL 2~8℃
I酶液12 mL 2~8℃
J底物(光敏感,避光保存)12 mL 2~8℃
K终止液(0.3M HCL)12 mL 2~8℃