Toxoplasma IgG ELISA

产品名称：Toxoplasma IgG ELISA
产    地：Demeditec 
产品货号：DETOX01
产品规格：96 Tests
产品说明：
Special remarks:
1. Intended Use
The DEMEDITEC Toxoplasma IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Toxoplasma in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of DEMEDITEC.
Monitoring of patients with engrafted organs as well as determination of antibodies in other body fluids were not validated and hence are within the responsibility of the user.
This assay is intended for in-vitro diagnostic use only.
Laboratory results can never be the only base of a medical report. The patient history and further tests have additionally to be taken into account.
2. General Information
Toxoplasmosis is an infection, which is caused by the parasite toxoplasma gondii. Up to 20% of the population carry the pathogenic agent, but only a few feel symptoms, because the immune system hinders the illness to break out. Pregnant women should however be very cautious, as an infection can be detrimental to the foetus.
Toxoplasma gondii belongs to the protozoae and is a parasite, which can infect many different species of mammals, amongst others human beings. Animals like cats, pigs and sheep spread oocysts as well as tissue cysts, which after ingestion by humans are converted into tachyzoites, and these can be found afterwards in nerve and muscle tissue. When a pregnant woman becomes infected, the tachyzoites can reach the foetus via the placenta.
The symptoms of toxoplasmosis are very different, and most of the infected persons do not really feel ill. A typical sign of the disease is a flu-like sensation with swollen lymph nodes and muscle pain, which may last for months. The severe form of toxoplasmosis causes damages to the brain, the eyes and further organs.
Even when the illness has ceased, a reactive chronic type can develop. The transmission of the disease takes primarily place by the ingestion of cat feces, e.g. during work in the garden or in the course of the pet care. But infective agents can also appear in raw meat or contaminated water.
Normally the clinical symptoms disappear after some weeks even without treatment, but for pregnant women and individuals with reduced immune system a therapy with drugs should be initiated.
The following laboratory methods are available: Complement fixation (CF), immunofluorescence (IFT) or ELISA. The determination of virus-specific IgM antibodies in fresh or reactivated infections is of special importance, the IgG test is used for the detection of immunity. If there is a severe connatal illness, an identification of the parasite out of peripheral blood, amniotic liquid or tissue samples can be tried.
3. Principle of the Test
The DEMEDITEC Toxoplasma IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Toxoplasma antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use calibrators are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Toxoplasma antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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