IGFBP-3 mouse/rat ELISA

产品名称：IGFBP-3 mouse/rat ELISA
产    地：Demeditec 
产品货号：DEE031
产品规格：96 Tests
产品说明：
Special remarks: Insulin-like growth factor binding protein 3, also known as IGFBP3, is a human gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Growth Hormone, Insulin-like Growth Factors and their binding proteins build up an endocrine system regulating not only longitudinal growth in humans but also influencing a broad variety of other physiological and pathophysiological processes like energy metabolism or tumor growth. Most effects of Growth Hormone (GH) are exerted by Insulin-like Growth Factors (IGF) mainly produced by the liver but also locally by specific tissues. The effects of IGF are also regulated, specific binding proteins (IGFBP 1-7) regulate bioavailability of IGF. After proteolytic cleavage of the binding proteins IGF is set free and able to bind to its receptor. The autophosphorylation of this thyrosine kinase receptor activates intra cellular signalling cascades. Some of these IGFBPs not only regulate the availability of IGF but also exert
IGF-independent effects on cell physiology.
IGFBP-3 ist the most abundant IGFBP in circulation and therefore of special relevance in regulation of IGF effects. This is reflected by the indicative value of serum IGFBP-3 concentration in diagnostics of growth disturbances. IGFBP-3 has also been shown to be able to induce apoptosis, promote tumor growth and inhibit cellular migration and metastasis dependent on tissue and tumor stage.
Intended use of this ELISA testkit:
This ELISA kit is suited for measuring IGFBP-3 in mouse and rat serum, cell lysates or nuclear extracts.
Performance characteristics and validation of this testkit:
This ELISA for mouse/rat IGFBP-3 (m/rIGFBP-3) is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The IGFBP-3 in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin-
Peroxidase conjugated second specific anti-mouse IGFBP-3-Antibody binds in turn to the immobilised mIGFBP-3. In the closing substrate reaction the turn of the colour will be high specific catalysed, quantitatively depending on the m/rIGFBP-3-level of the samples.
The standards of this ELISA are recombinant mouse IGFBP-3 in concentrations of 0.078, 0.156, 0.313, 0.625, 1.25, 2.50 ng/ml and 5 ng/ml.
Sensitivity
The analytical sensitivity of this ELISA yields 0.018 ng/ml (2 SD of zero standard in 21fold determination).
The Inter- and Intra-Assay variation coefficients were found less than 8.36 % and 4.36%.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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