IGF-I mouse/rat ELISA

产品名称：IGF-I mouse/rat ELISA
产    地：Demeditec 
产品货号：DEE025
产品规格：96 Tests
产品说明：
Special remarks:
Measurement of IGF-I in serum of mice and rats.
Beside different cell culture models and studies with human patients, mice and rats are suitable model organisms for basic research and pre-clinical studies. Thus, we developed this test system as a tool for IGF-I measurements in mice and rat for usage in research and preclinical studies. Even if the comparability of mice and humans is limited we offer some background information on the human IGF-I system in the following section:
Insulin-like growth factors (IGF) I and II play a pivotal role in regulating the proliferation and differentiation of many cell types. IGF-I is identical with Somatomedin C (Sm-C) and has a molecular weight of 7649 daltons. Its major regulators are growth hormone (GH) and nutrition. In contrast to many other peptide hormones, IGFs are avidly bound to specific binding proteins (IGFBP). The seven IGFBPs which are known at present either bind IGF-I and IGF-II with similar affinities or show a preference for IGF-II. A major problem of IGF-I measurement results from the interference of IGFBPs in the assay. Therefore, various techniques were applied to physically separate IGF-I from its binding proteins before measurement, including (a) size exclusion chromatography under acidic conditions, (b) solid-phase extraction and (c) acid-ethanol extraction. These techniques, however, are either inconvenient or time-consuming or give incomplete and not reproducible recoveries. The most widely used method is the acid-ethanol extraction with a recovery of only 70-80 % of IGFBP-bound IGF-I as a result of co-precipitation. The absolute results of such an extraction are therefore false low. The extraction removes the IGFBPs only insufficiently and leads to reduction in sensitivity of the assay due to predilution of the samples by the extraction procedure. Furthermore, the remaining IGFBP may still interfere in the assay. In addition, the acid-ethanol extraction is ineffective in specimens other than serum or plasma (e.g. cell culture media), in which determination of IGF-I is already difficult enough due to the fact that IGFBPs are frequently present at large excess.
To avoid these difficulties, an uncomplicated assay was developed, in which special sample preparation is not required before measurement.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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