IGFBP-1 ELISA

产品名称：IGFBP-1 ELISA
产    地：Demeditec 
产品货号：DEE001
产品规格：96 Tests
产品说明：
Special remarks: Besides serum and Heparin- or EDTA plasma the following body liquids can be used:
Amniotic fluid:dilution 1:8000
Breast milk:dilution  1:10
Urine:dilution  1:2.5
Saliva:dilution  1:2.5
Bronchial lavage:dilution  1: 2.5
Sputum:dilution  1:20
Cell culture media:dilution  1:5 or more
Results in citrate plasma are about 15% reduced. Slight hemolysis of the samples does not disturb the determination.
The Insulin-like Growth Factors I and – II are free in body fluids and tissues but are bound to specific binding proteins. Until today seven different binding proteins (IGFBP-1 to –7) can be differentiated additionally several IGFBP-related proteins have also been detected.  Bioavailability of IGF is regulated by these IGFBPs or better their proteolytic cleavage which reduces affinity to IGF. But the IGFBPs as well as their proteolytic fragments can also exert IGF-independent effects, like influencing cell migration or proliferation.
IGFBP-1 (Placental Protein 12) consists of 234 aminoacids and has a molecular weight of approximately 25kDa. The coding DNA region is located on chromosome 7. IGFBP-1 is mainly synthesized by foetal and ***** liver tissue and decidual endometrium. Intensity of Expression varies enduring menstruation with a maximal expression in the late secretory phase. Further IGFBP-1 expression seems to be regulated by Insulin concentration, with Insulin inhibiting the expression. Insulin regulation results in diurnal fluctuations of up to factor 10. IGFBP-1 is posttranslational modificated by phosphorylation of serine residues 101, 119 and 169.  Phosphorylation has physiological relevance as it increases affinity of IGFBP-1 to IGF. In ***** humans phosphorylated IGFBP-1 of the liver is the predominant form in circulation. IGFBP-1 produced by endometrial tissue is significantly less phosphorylated than the liver originated form.
In pregnancy IGFBP-1 maternal serum concentration increases significantly with maximal values in the second trimester or 22-23 week of gestation (75.8 ng/ml) and decreases slowly until term. IGFBP-1 concentration are not only increased in maternal but also in foetal serum and with extremely high concentrations in amnion fluid. Here concentration can reach more than the 1000-fold of serum values. Long-term changes of serum IGFBP-1 concentration can also be found in amnion fluid: IGFBP-1 level of the child decreases after birth until it reaches the low steady-state level of puberty and *****hood. 
Short term IGFBP-1 serum concentration is strongly influenced by nutrition level and therewith by insulin. Decreasing IGFBP-1 levels can be found enduring fasting or in diabetes; IGFBP-1 levels increase in case of intensive exercises.
Relevance of serum and amnion IGFBP-1 in diagnostics has been investigated in several areas. A diagnostic value was assigned for trisomy 18, intrauterine growth retardation, endometrial tumors and pre-eclampsia.
Thoroughly investigated was the diagnostic value in insulin resistance and pre-term rupture of the membrane and specially in the second field a significant diagnostic value could be demonstrated.
Energy metabolism
Based on the influence of Insulin on IGFBP-1 serum concentrations IGFBP-1 is said to be a possible marker for insulin resistance. Because measurement of IGFBP-1 is much easier facilitated than Glucose – uptake rate this would simplify diagnosis of insulin resistance.
In a small study Maddux  et al were able to demonstrate with 23 non-diabetic patients, that IGFBP-1 serum concentration correlated very well with Glucose-uptake rate, even better than the HOMA index does.
Pregnancy
 In pregnancy a significant difference in IGFBP-1 serum concentration of  healthy pregnant and diabetic and pre-eclamptic women was found (102,8 vs. 203,71 or 281,09 ng/ml respectively).
 Also the evaluation of IGFBP-1 as marker for membrane rupture showed a high specificity (97%) and sensitivity (75%) of IGFBP-1 in vaginal/cervical secrets. In case of intact membrane IGFBP-1 concentration was < 90ng/ml in the secretion. Enduring 8 hours after spontaneous or induced membrane rupture IGFBP-1 values increased significantly with a median concentration of 1900 ng/ml. In this study IGFBP-1 concentrations von >100ng/ml were set as threshold for detection of amnion fluid and therewith diagnosis of membrane rupture. A positive predictive value of 97% clearly shows that IGFBP-1 is a suitable marker for premature membrane rupture.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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