Involucrin specific components

产品名称：Involucrin specific components
产    地：Demeditec
产品货号：DEBT650
产品规格：24x96 Tests
产品说明：
Special remarks: This is not a complete kit; it just contains the specific compomemts and has to be completed with commercial reagents.
Involucrin is a recently recognized structural component of mature squamous epithelial cells. We examined involucrin expression using an immunoperoxidase technique in normal skin and in a variety of epidermal hyperplasias and neoplasms to determine whether distinctive staining patterns existed within these lesions. Four patterns of reactivity were observed: diffuse intracellular staining typical of keratinocytes of the upper third of normal epidermis and epidermal hyperplasias and benign neoplasms; staining at cell borders, seen principally in benign epidermal neoplasms; patchy staining characteristic of squamous cell carcinoma in situ; and absence of staining in benign and neoplastic basaloid epithelium. Invasive nests of squamous cell carcinomas were negative for involucrin reactivity, whereas pseudoinvasive tongues of epithelium at the bases of keratoacanthomas were focally positive. These results suggest that immunoperoxidase staining for involucrin may be useful in distinguishing certain benign from malignant epidermal neoplasms as well as in understanding the altered maturation and kinetics of proliferative processes afflicting keratinocytes.
Involucrin, a soluble cytoplasmic protein with a highly repetitive amino acid sequence, serves as a substrate for transglutaminase cross-linking in the formation of cornified envelopes.  Like the cross linked envelope, involucrin expression is a specific marker for keratinocyte differentiation.  This protein is synthesized not only by cells of the epidermis and other squamous epithelia, but also in epithelia undergoing squamous metaplasia and in certain tumors, as in the lung, which display epidermoid characteristics.  In culture, the growth and differentiation of keratinocytes, especially those derived from tumors, can be modulated greatly by  physiological (retinoids, calcium) and toxic agents (including  carcinogens). The involucrin content of the cells is highly responsive to such treatments and thus its measurement by ELISA can serve as a convenient and sensitive indicator of the cellular response.
This Involucrin ELISA Kit is designed to provide a reproducible, quantitative method of measuring Involucrin. The assay is highly sensitive, 0.05-0.5 nanograms per well.  Levels of Involucrin can be directly measured after separation of cellular debris.  The kit includes purified human Involucrin, a highly specific rabbit anti-human Involucrin and an affinity purified goat anti-rabbit IgG alkaline phosphatase.  Sufficient quantities of these reagents are provided for approximately 24 microtiter plates.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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