Osteocalcin mouse

产品名称：Osteocalcin mouse
产    地：Demeditec
产品货号：DEBT470
产品规格：96 Tests
产品说明：
Special remarks: Osteocalcin, the vitamin K-dependent protein of bone, is a specific product of the osteoblast.  It is distinguished by its small size (5800 daltons) and the presence of gamma-carboxy-glutamic acid (Gla).  In the presence of ionic calcium, the Gla residues allow a specific conformational change in the protein, which in turn promotes osteocalcin binding to bone mineral and subsequent accumulation in bone matrix.  While osteocalcin is primarily deposited into the extracellular matrix of bone, a small amount can be detected in the blood.  Circulating osteocalcin is thought to reflect that portion of newly synthesized protein that does not bind to bone but is released directly into the circulation.
This sandwich ELISA Kit is specific for mouse osteocalcin only.  Both carboxylated and decarboxylated mouse osteocalcin are recognized.  Mouse osteocalcin can be measured directly from serum, heparinized plasma, bone extracts and cell culture supernates.  A polyclonal antibody directed against the N-terminus is bound to the polystyrene wells.  An overnight incubation is done with sample and biotinylated antibody specific for the C-terminus.  Detection is achieved after washing and a short incubation with Peroxidase-Streptavidin Conjugate.Standards of highly purified Mouse Osteocalcin are used to generate a standard curve.
The design of the animal experiment is most important. Many variables effect serum osteocalcin levels: age, growth rate, hormonal status, vitamin D intake, stress, circadian rhythm, etc. It is desirable to take blood samples under the same conditions: time of day, without stress, etc. and process the serum (or plasma) the same way for each sampling. We have found a range of 40-150 ng/ml in mouse serum that we evaluated. Young mice (<60 days) can have levels of 500ng/ml or more.
Concentrations of osteocalcin in conditioned media range from <1ng/ml to 400ng/ml depending on the cell type and culture conditions. Full dilution series should be done to establish linearity of dilution.  Medium and serum interference should be assessed by appropriate blanks and internal standards of medium with added mouse osteocalcin. Fetal Bovine Serum shows little interference. Other species of sera should be tested. Culture media can generally be assayed directly.
This test also allows the measurement of osteocalcin in EDTA extarcts of bone powder after appropriate dilution. Typical levels are 1 - 2 ng per microgram of dry bone powder.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!