ANCA Screen ELISA

产品名称：ANCA Screen ELISA
产    地：Demeditec 
产品货号：DE7050
产品规格：96 Tests
产品说明：
Special remarks:
The DEMEDITEC ANCAScreen assay is a qualitative enzyme immunoassay (EIA) intended to screen for the presence of IgG class autoantibodies against PR3 and MPO in human serum or plasma as an aid in the diagnosis of systemic vasculitides.
Anti-neutrophil cytoplasmic antibodies (ANCA) represent a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of the ANCAs are the immunofluorescent methods. With indirect immunofluorescence techniques two main patterns are recognized.
- a cytoplasmatic (c-ANCA) pattern  - and a perinuclear (p-ANCA) type
Recently, the main antigens for the c- and p-ANCAs have been identified. The target antigen for 80-90 % of c-ANCA antibodies is the proteinase 3 (PR3), a serine proteinase from a-granules.
10-20 % of c-ANCAs are directed to other proteins.
The solubilization of ethanol-fixed granulocytes causes a binding of positively charged proteins around the nucleus. Antibodies to these proteins appear in the immunofluorescence as p-ANCAs.
Approx. 90 % of p-ANCA positive sera contain autoantibodies directed to myeloperoxidase (MPO) which is located in the granula of neutrophilic granulocytes. Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin G and lysozyme often result in a similar p-ANCA patterns. Beside different untypical variants of p-ANCA, IF patterns of granulocyte specific antinuclear antibodies (GS-ANA) are indistinguishable from those of p-ANCAs. A distinct interpretation and classification of the IF patterns is quite difficult. Therefore every positive IF-ANCA finding esp. p-ANCAs should be differentiated by ELISA techniques using purified antigens.
The anti-PR3 antibody titer correlates well with the clinical status of the disease. Antibody titers are decreasing under therapy and become negative after remission. Anti-MPO levels correlate with the clinical status too. They are always higher during the active disease than after remission.
Anti-PR3 and anti-MPO antibodies are reliable serological markers for the diagnosis of vasculitides. PR3 is the classical autoantigen in Wegener´s granulomatosis with a clinical specificity of more than 95%. p-ANCAs are documented to be present in 70% of patients with Microscopic Polyangiitis.
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