Neopterin ELISA

产品名称：Neopterin ELISA
产    地：Demeditec 
产品货号：DE65101
产品规格：96 Tests
产品说明：
Special remarks:
SUMMARY AND EXPLANATION
Pteridines constitute a large and structurally varied group of natural compounds involved in the biosynthestic pathways of cofactors and vitamins (Fig. 1). Derivatives with the structure 2-amino-4-oxo are designated by the term "pterins" (Fig. 1) and can be divided into two groups:
Conjugated Pterins
Those derived from folic acid, containing a p-aminobenzoate plus glutamate
Unconjugated Pterins
Contain neither of these two groups, instead, a substitution occurs mainly at the 6-position of the ring nucleus
Neopterin [D-erythro-neopterin], a 253.2 Da low-molecular-mass molecule, belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosinetriphophate (GTP). The enzyme GTP-cyclohydrolase-I catalyses this reaction in monocytes and macrophages. Neopterin is excreted by activated macrophages, and can be determined in serum, plasma and other body fluids by immunoassay.
Neopterin serves as a marker of cellular immune system activation, and it can be used as a prognostic predictor for certain types of diseases. Neopterin levels provide an especially valuable indicator of clinical activity in diseases characterised by acute changes in severity.
The determination of neopterin levels in human body fluids offers a useful and innovative tool for monitoring diseases associated with the activation of cell-mediated immunity such as viral infections and graft rejection in transplantation. In addition, blood samples are usually not tested for all possible infections. Therefore, the measurement of neopterin in blood donor samples is a useful tool in order to reduce the risk of infections via blood transfusion.
Neopterin concentrations may be significantly increased in other particular disease state compared to controls and serial measurements of neopterin concentrations in a particular patient may be useful in monitoring the course of a condition. Some potential diagnostic applications for the determination of neopterin are:
-follow-up of traumatized ICU patients
-prognostic indicator in HIV infections
-early indication of graft-vs-host disease in bone-marrow transplant patients
-early indication of episodes of graft rejection.
-indicator of disease activity in autoimmune diseases
-diagnosis of viral infections
-differential diagnosis of acute viral and bacterial infections
-prognostic indicator in malignant diseases
-follow-up control of chronic infections
-monitoring of immunostimulatory therapy
PRINCIPLE OF THE TEST
The Neopterin assay is a competitive enzyme immunoassay for the quantitative determination of neopterin in serum, plasma and urine using high affinity monoclonal antibody specific for neopterin. An unknown amount of antigen (neopterin) in the sample and a fixed amount of enzyme labelled antigen (neopterin bound horse radish peroxidase) compete for the antibody-binding sites (mouse monoclonal antibody against neopterin). Both antigen-monoclonal antibody complexes bind to the wells of the microtiter strips coated with a goat-anti-mouse antibody. Unbound antigen and conjugate (neopterin bound horse radish peroxidase) are removed by washing. The intensity of the color developed after the substrate incubation is directly proportional to the amount of bound conjugate and inversely proportional to the amount of neopterin in the standard or sample. Therefore, as the concentration of neopterin in the sample or standard increases, the intensity of the blue color will decrease. An acidic stop solution is added which changes the chromogen color from blue to yellow. The microwells are measured optically by a microplate reader with an absorbance filter of 450nm (OD450). The optical densities of the samples are compared to the OD’s of the kit standards and an interpretative result is determined. The results are calculated by plotting a Standard curve (optical density versus concentrations of neopterin standard), from which the neopterin concentrations in the patient samples can be read off directly.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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