5a-Dihydrotestosterone (DHT) RIA

产品名称：5a-Dihydrotestosterone (DHT) RIA
产    地：Demeditec 
产品货号：DE5170
产品规格：96 Tests
产品说明：
Special remarks:
Intended use
For in vitro determination of serum or plasma DHT levels.
5a-Dihydrotestosterone (DHT; 17ß-Hydroxy-5a-androstan-3-one), the most potent naturally-occurring androgen, is produced from testosterone through the action of cholestenone 5a-reductase. The concen­­­trations of 5a-reductase are highest in certain peripheral tissues, including genital skin and hair follicles, and is localized intracellularly in apparent association with the nuclear membrane. DHT exerts its biological action by intracellular binding to the androgen receptor; this complex is then trans­ferred to the nucleus where DNA-binding occurs with resultant effects on DNA transcription [2]. Most of the residual DHT undergoes intracellular metabolism to 3a-androstanediol and 3a-andro­stane­diol glucuronide. Only a small proportion of DHT escapes into the peripheral circulation, where it is present primarily complexed to sex-hormone binding globulin. Testosterone causes virilization of the Wolffian ducts during fetal life, while DHT is responsible for the development of the male external genitalia and prostate, and is primarily responsible for the physi­cal changes which occur during male sexual maturation. An autosomal-recessive genetic defi­cien­­cy of 5a-reductase, sometimes called male pseudohermaphroditism or pseudovaginal perineo­scrotal hypo­spadias, leads to inadequate differentiation of DHT-dependent peripheral tissues. Male infants with this disorder have ambiguous genitalia and are often raised as females, although signi­fi­cant virilization may occur later in life presumably due to the natural increase in testosterone levels. Measurement of DHT concentrations can be complicated by antibody cross-reactivity to testos­terone. The DHT Double Antibody radioimmunoassay utilizes a sample oxidation / extrac­tion procedure to remove most of the testosterone, coupled with a specific immunoassa.
Principle of the method
The procedure follows the basic principle of radioimmunoassay where there is competition between the radioactive and non-radioactive antigen for a fixed number of antibody binding sites. The amount of (I-125)-labeled analyte bound to the antibody is inversely proportional to the concentration of unlabeled analyte present. The separation of free and bound antigen is achieved by using a double antibody system.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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