CA 72-4 ELISA

产品名称：CA 72-4 ELISA
产    地：Demeditec 
产品货号：DE5071
产品规格：96 Tests
产品说明：
Special remarks:
1 Introduction
1.1 Intended Use
The DEMEDITEC CA 72?4 ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of CA 72?4 (TAG-72) in serum and plasma.
1.2 Summary and Explanation
CA 72?4 (Cancer antigen 72-4) was originally described as an antigenic determinant recognized by B 72.3, a murine monoclonal antibody raised against a membrane extract of mama carcinoma metastases (1). CA 72?4 was identified as a 1 MDa mucine-like Glycoprotein complex termed TAG-72 (tumor associated antigen 72) (2). The molecular weight of the TAG-72 protein is 48 kD. Elevated CA 72?4 levels in serum and plasma have been reported in various malignant diseases including carcinomas of pancreas, stomach, gall, colon, mamma, ovaries, cervix and endometrium (3). The highest diagnostic sensitivities are found for carcinomas of the gastrointestinal tract and ovaries. Although some benign diseases such as rheumatic diseases or ovary cysts may also result in elevated levels of CA 72?4, clinical studies demonstrated diagnostic specificities of more than 95% for gastrointestinal and ovarian malignancies (4). There is a good correlation between CA 72?4 levels and tumor stage and size (3). CA 72?4 is the marker of choice for the therapeutic monitoring and follow-up care of gastrointestinal cancer patients. Suitable second markers are CA 19-9 or CEA. Additionally, CA 72?4 has been used as an independent marker for the therapeutic monitoring and follow-up care of ovarian cancer patients, in particular in CA 125 negative patients (3, 5).
2 Principle of the test
The DEMEDITEC CA 72?4 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.
The microtiter wells are coated with a monoclonal mouse antibody (Clone CC49) directed towards a unique antigenic site on a CA 72?4 molecule.  An aliquot of patient sample containing endogenous CA 72?4 is incubated in the coated well with enzyme conjugate, which is an anti-CA 72?4 antibody (Clone B72.3) conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase is proportional to the concentration of CA 72?4 in the sample.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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