Hepcidin Prohormone ELISA

产品名称：Hepcidin Prohormone ELISA
产    地：Demeditec 
产品货号：DE4644
产品规格：96 Tests
产品说明：
Special remarks:
1 Introduction
The DEMEDITEC Hepcidin Prohormone Enzyme Immunoassay Kit provides materials for the quantitative determination of Hepcidin Prohormone in human serum and urine.
This assay is intended for research use only.
Hepcidin is a small cystein-rich peptide produced in the liver [1]. This molecule regulates the absorption of iron in the body similarly to the correlation of insulin and sugar.
Hepcidin was initially isolated as a 25 amino acid (aa) peptide in human plasma and urine exibiting antimicrobial activity [2,3].
Human Hepcidin is produced from a 84 aa precursor including a putative (24 aa) signal peptide.
Application of the present ELISA allows the detection and determination of Hepcidin Prohormone (Pro?Hepcidin) in serum of patients suffering from several disorders of iron metabolism.
2 Principle of the test
The DEMEDITEC Hepcidin Prohormone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Hepcidin Prohormone molecule (28-47 aa). Endogenous Hepcidin Prohormone of a patient sample competes with a Hepcidin Prohormone-biotin conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound biotin conjugate is reverse proportional to the concentration of Hepcidin Prohormone in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Hepcidin Prohormone in the patient sample.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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