Proinsulin rat, mouse

产品名称：Proinsulin (rat, mouse)
产    地：Demeditec 
产品货号：DE4609
产品规格：96 Tests
产品说明：
Special remarks:
Intended use
Rat/Mouse Proinsulin ELISA provides a method for the quantitative determination of proinsulin in rat and mouse serum, plasma, cell culture medium and cellular extracts.
Summary and explanation of the test
Proinsulin is the precursor of insulin, which is the principle hormone responsible for the control of glucose metabolism. Proinsulin is synthesized in the ß-cells of the islets of Langerhans and is subsequently processed to form C-peptide and insulin. In most species the insulin gene exists in a single copy. Rats and mice however, have two closely related genes which produce two nonallelic insulins, insulin I and insulin II (1). In rats, insulin I is more abundant than insulin II, whereas the opposite is found in mice (2). It is suggested that the difference in ratio between insulin I/insulin II may depend both on differences in expression of proinsulin I and proinsulin II, and in the rates of conversion to insulin (3). Increased proinsulin over insulin ratio may be found in rat and mouse models of hyperglycemia (4,5), or after manipulation of proinsulin-processing enzymes (6,7). The Rat/Mouse Proinsulin ELISA (EIA?4609) is specific for proinsulin and does not cross-react with insulin or c-peptide in rat or mouse.
Principle of the procedure
Rat/Mouse Proinsulin ELISA is a solid phase two-site enzyme immunoassay. It is based on the sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the proinsulin molecule. During incubation, proinsulin in the sample reacts with anti-proinsulin antibodies bound to the microtitration well and peroxidase-conjugated anti-proinsulin antibodies simultaneously. After a simple washing step that removes unbound sample and enzyme labelled antibody, the bound conjugate is detected by reaction with 3,3´-5,5´-tetramethylbenzidine (TMB). The reaction is stopped by the addition of acid, giving a colorimetric endpoint that can be read spectrophotometrically.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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