hCG RIA

产品名称：hCG RIA
产    地：Demeditec 
产品货号：DE45100
产品规格：100 Tests
产品说明：
Special remarks:
1.Description
The 125I?hCG RIA system provides direct quantitative in vitro determination of human Chorionic Gonadotrophin (hCG) in human serum. hCG can be assayed in the range of 0?250 IU/ml using 25 µl serum samples.
2.Introduction
hCG appears in the sera of pregnant women five days after the implantation of blastocyst and its concentration continually increases until the third month of the pregnancy. The maximum concentration can reach values up to 100 IU/ml. Then the hormone level drops to 25 IU/ml and stays around this value until the last trimester.
Elevated hCG concentrations can be seen in the case of trophoblastic and nontrophoblastic neoplasia, and choriocarcinoma.
This kit can  be used in the risk assessment  of Down’s Syndrome (Trisomy 21) in combination with other biochemical and ultrasound parameters as specified above, taking also into account other data like maternal age and weight and using a validated software for Down’s Syndrome risk assessment:
1.Triple test: maternal serum AFP, hCG and unconjugated Estriol determination in the second trimester of pregnancy.
2.Quadruple test: maternal serum AFP, hCG, unconjugated Estriol and Inhibin-A determination in the second trimester of pregnancy.
3.Integrated test: maternal serum PAPP-A determination and nuchal translucency (NT) thickness measurement by an ultrasound scan in the first trimester of pregnancy and maternal serum AFP, hCG, unconjugated Estriol and Inhibin-A determination in the second trimester.
3.Principle of method
This assay is based on the competition between unlabelled hCG and fixed quantity of 125I-labelled hCG for limited number of binding sites on hCG specific antibody. Allowing to react a fixed amount of tracer and antibody with different amounts of unlabelled ligand the amount of tracer bound by the antibody will be inversely proportional to the concentration of unlabelled ligand.
During 1-hour of incubation period with continuous agitation, immuno-complex is immobilized on the reactive surface of test tubes. After incubation the reaction mixture is discarded, and the radioactivity is measured in a gamma counter.
The concentration of antigen is inversely proportional to the radioactivity measured in test tubes. By plotting binding values against a series of calibrators containing known amount of hCG, a calibration curve is constructed, from which the unknown concentration of hCG in patient samples can determined.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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