C-Peptide IRMA

产品名称：C-Peptide IRMA
产    地：Demeditec
产品货号：DE35100
产品规格：100 Tests
产品说明：
Special remarks:
The C-PEPTIDE [125I] IRMA system provides a direct quantitative determination of C-peptide in human serum and urine. C-peptide can be assayed in the range of 0?30ng/ml (in human serum) and 0-300 ng/ml (in urine) using 50 µl serum samples. Each kit contains materials sufficient for 100 assay tubes permitting the construction of one standard curve and the assay of 42 unknowns in duplicate. Introduction C-peptide (connective peptide) is a polypeptide with a molecular weight of 3600,containing 31 amino acid. It derives from proinsulin produced in pancreatic beta cells. Proinsulin is converted to insulin and C-peptide, which are then secreted into the portal blood in an equimolar quantity. Contrary to insulin, C-peptide is not extracted by the liver, but enters the systemic circulation. In the diagnosis of diabetes, C-peptide is a more reliable indicator of insulin secretion than is the insulin itself. Further­more, the concentration of C-peptide is not affected by inter­ference from insulin antibodies often present in patients receiving insulin therapy. The assessment of residual endo­genous insulin secretion by the measurement of C-peptide is an indispensable tool for the diagnosis and treatment of diabetes mellitus.
Principle of method
The technology uses two high affinity monoclonal antibodies in an immuno- radiometric assay (IRMA) system. The 125I labelled signal-antibody binds to an epitope of the C-peptid molecule, which is different from that recognised by the unlabelled capture-antibody. The two antibodies react simul­taneously with the antigen present in standards or samples which leads to the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”. During a 3-hour incubation period with continuous agitation immuno-complex is immobilised on the reactive surface of test tubes. Reaction mixture is then discarded, test tubes are washed exhaustively, and the radioactivity is measured in a gamma counter. The concentration of antigen is directly proportional to the radio­activity measured in test tubes. By constructing a calib­ration curve plotting binding values against a series of standards containing known amount of C-peptid, the unknown concentration of C-peptid in patient samples can determined.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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