Vasopressin ELISA

产品名称：Vasopressin ELISA
产    地：Demeditec
产品货号：DE3122
产品规格：96 Tests
产品说明：
Special remarks:
1 DESCRIPTION
The DEMEDITEC Vasopressin ELISA kit is a competitive immunoassay for the quantitative determination of Vasopressin in samples. The kit uses a polyclonal antibody to Vasopressin to bind, in a competitive manner, the Vasopressin in the sample or an alkaline phosphatase molecule which has Vasopressin covalently attached to it. After a simultaneous incubation at 4ºC the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Vasopressin in either standards or samples. The measured optical density is used to calculate the concentration of Vasopressin. For further explanation of the principles and practice of immunoassays please see the excellent books by Chard or Tijssen.
2 INTRODUCTION
Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei.
It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions.
AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into
peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication.
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