FSH IRMA

产品名称：FSH IRMA
产    地：Demeditec
产品货号：DE31100
产品规格：100 Tests
产品说明：
Special remarks:
Introduction
The Follicle Stimulating Hormone (Follitropin or hFSH) is a glycoprotein with a molecular weight of 30000, secreted by the adenohypophysis. Like other glycoprotein hormones (LH, TSH and HCG), hFSH contains two different subunits, an a-and a b-chain, linked by noncovalent bounds. The primary structures of the a subunits of hFSH and of those mentioned are virtually identical, whilst their b subunits are different. The b subunits are responsible for the immunological and biological specificity of these hormones. The hFSH synthesis and release is stimulated by the hypothalamic Gonadotrophin-Releasing Hormone (GnRH), whereas the ovarian steroids secreted from the corpus luteum control further secretions of hFSH by negative feed-back. The measurement of hFSH concentrations is an important part of the investigation of disorders of the hypothalamic-pituitary-gonadal axis. It is recommended to measure both hFSH and hLH to discriminate between hypothalamic and pituitary dysfunction.
Principle of method
The technology uses two high affinity monoclonal antibodies in an immunoradiometric assay (IRMA) system. The 125I labelled signal-antibody binds to an epitope of the FSH molecule spatially different from that recognized by the biotin-capture-antibody. The two antibodies react simultaneously with the antigen present in standards or samples, which leads to the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”. During a 2-hour incubation period with shaking immuno-complex is immobilized to the reactive surface of streptavidin coated test tubes. Reaction mixture is then discarded, test tubes washed exhaustively, and the radioactivity is measured in a gamma counter. The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of calibrators containing known amount of hFSH, the unknown concentration of hFSH in patient samples can determined
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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