Cortisol RIA

产品名称：Cortisol RIA
产    地：Demeditec 
产品货号：DE28100
产品规格：100 Tests
产品说明：
Special remarks:
Introduction
Cortisol is the main glucocorticoid which is produced by the adrenal cortex. It regulates carbohydrate, protein, fat and purine metabolism, electrolyte and water balance. Cortisol has a role in blood pressure regulation and resolution of inflammation. The determination of cortisol levels can be used for diagnosing the functional disturbances of the hypothalamus-pituitary-adrenal cortex (HPA) axis. In normal individuals cortisol secretion has a characteristic rhythm: the cortisol level is the highest in the morning, by the evening it decreases to approximately half that level.
The above test kit measures the total (unconjugated) Cortisol in serum. There is also a method available for the measurement of free Cortisol in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DES6611.
Principle of the method
This assay is based on the competition between unlabelled cortisol and fixed quantity of 125I-labelled cortisol for limited number of binding sites on cortisol specific antibody. Allowing to react a fixed amount of tracer and antibody with different amounts of unlabelled ligand, the amount of tracer bound by the antibody will be inversely proportional to the concentration of unlabelled ligand. During a 2-hour incubation period with continuous agitation, immuno-complex is immobilized on the reactive surface of test tubes. After incubation the reaction mixture is discarded, and the radioactivity is measured in a gamma counter. The concentration of antigen is inversely proportional to the radioactivity measured in test tubes. By plotting binding values against a series of calibrators containing known amounts of cortisol, a calibration curve is constructed, from which the unknown concentration of cortisol in patient samples can determined.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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