Estrone ELISA

产品名称：Estrone ELISA
产    地：Demeditec
产品货号：DE4174
产品规格：96 Tests
产品说明：
Special remarks:
Intended Use
The DEMEDITEC Estrone ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Estrone in serum and plasma.
Summary and Explanation
Estrone (3-hydroxy-1,3,5 (10)-estratrien–17-one) is beside estradiol and estriol one of the three major naturally occuring estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Bioassay data indicate that the estrogenic activity of estrone is considerably lower in comparison to estradiol. However, the physiological role of endogenous estrone is not well defined. Estrone is produced primarily from androstenedione. In premenopausal women, more than 50% of the estrone is secreted by the ovary. In prepubertal children, men and postmenopausal women, the major portion of estrone is derived from peripheral tissue conversion. During the follicular phase of the menstrual cycle the estrone level inreases with a clear peak around day 13. The peak is of short duration and by day 16 of the cycle levels will be low again. A second peak during the luteal phase occurs around day 21 of the cycle. If fertilization does not occur production of estrone decreases again. These changes of estrone concentration are in parallel to that of estradiol. Until the 4. to 6. week of pregnancy, estrone originates primarily from maternal sources such as the ovaries, adrenals, or peripheral conversion thus remaining within the normal values. After the 6. to 10. week of pregnancy the values increase gradually due to placental secretion of estrone. After menopause, estrone levels do not decline as dramatically as estradiol levels. In postmenopausal women estrone is the major estrogen. In males the concentration of E1 has been reported to rise up with age inversely to that of 17-OH-progesterone. In premenopausal women excessive estrone levels can result from the conversion of large amounts of androstenedione produced in polycystic ovary syndrom and ovarian tumors.
Principle of the test
The DEMEDITEC Estrone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with streptavidin and biotinylated Estrone molecules. Endogenous Estrone of a patient sample competes with this immobilized Estrone for binding sites of a HRP-conjugated sheep antibody directed towards an antigenic site of estrone. After incubation the unbound antibody conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Estrone in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Estrone in the patient sample.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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