hCG-b, free IRMA

产品名称：hCG-b, free IRMA
产    地：Demeditec 
产品货号：DE41100
产品规格：100 Tests
产品说明：
Special remarks:
Introduction
Human Chorionic Gonadotrophin (hCG) is a glycoprotein with a molecular weight of 38000, secreted by the trophoblast cells of placenta. It contains two different subunits. The a-subunit is common to all glycoprotein hormones and the ß-subunit is responsible for the immunological and biological specifity. Free form of both subunits are present in the circulation. Normal pregnancy is associated with an exponential increase of both holo-hCG and its free ß-subunit. hCG appears in the sera of pregnant women 5 days after the implantation of blastocyst and around the 10th week of pregnancy its concentration can reach up to 100 mIU/ml. The significant elevation of free ßhCG in maternal serum is now considered as a specific and sensitive diagnostic marker of gestational trophoblastic neoplasiae (GTN) including hydatidiform mole (both invasive and noninvasive) and choriocarcinoma. In addition to its use as a screening method, the appearence of free ßhCG after surgical abortion is a good indication of tumour recurrance. Free ßhCG has been proposed as one of the multi-parametric screening panel of Down syndrome, since this genetic disorder is associated with an extremely high concentration of free ßhCG of maternal serum in the first trimester. The ectopic production of free ßhCG is reported in a number of adenocarcinomas, in metastatic breast cancer and in testicular cancer.
Principle of method
The technology uses two monoclonal antibodies of high affinity in an immunoradiometric assay (IRMA) system. It offers an increased level of sensitivity and specificity compared with conventional RIA methods. This assay is based on a two-steps procedure to eliminate interference from serum protein. In the first stage the serum sample is incubated in streptavidin coated tubes with biotin labelled monoclonal antibody (capture). During an 1-hour incubation period with continuous agitation the immuno-complex is developed and immobilized on the reactive surface of test tubes. After incubation tubes are washed. In the second stage 125I labelled monoclonal antibody (signal) is added and it binds to an epitope of the free ßhCG molecule different from that recognised by the un­labelled capture-antibody developing the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”. Reaction mixture is then discarded, test tubes washed exhaustively, and the radioactivity is measured in a gamma counter. The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of calibrators containing known amount of free ßhCG, the unknown concentration of free ßhCG in patient samples can be determined.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!