Estriol unconjugated RIA

产品名称：Estriol unconjugated RIA
产    地：Demeditec 
产品货号：DE40100
产品规格：100 Tests
产品说明：
Special remarks:
Introduction
Estriol is one of the most important steroid hormones in women. Estriol is almost exclusively produced during pregnancy, originating from a precursor synthesized by fetal adrenal glands and then converted to estriol by fetal liver and placenta. In the maternal liver, estriol is conjugated to form sulphates and glucoronides, which are then excreted in the urine with a half-life of app. 20 minutes. In the maternal circulation unconjugated estriol accounts for app. 10% of total estriol. Unconjugated estriol concentration rises during pregnancy, being 2 - 3 orders of magnitude higher in the final trimester. During this period, a rapid decrease in estriol levels is indicative of fetal distress. This kit can  be used in the risk assessment  of Down’s Syndrome (Trisomy 21) in combination with other biochemical and ultrasound parameters as specified above, taking also into account other data like maternal age and weight and using a validated software for Down’s Syndrome risk assessment:
1.Triple test: maternal serum AFP, hCG and unconjugated Estriol determination in the second trimester of pregnancy.
2.Quadruple test: maternal serum AFP, hCG, unconjugated Estriol and Inhibin-A determination in the second trimester of pregnancy.
3.Integrated test: maternal serum PAPP-A determination and nuchal translucency (NT) thickness measurement by an ultrasound scan in the first trimester of pregnancy and maternal serum AFP, hCG, unconjugated Estriol and Inhibin-A determination in the second trimester.
Principle of method
This assay is based on the competition between unlabeled Estriol in standards and samples and a fixed quantity of 125I-labelled Estriol for a limited number of binding sites on Estriol-specific antibody. During incubation the antigen-antibody immuno-complex is immobilized on the reactive surface of test tubes, afterwards the reaction mixture is discarded and the radioactivity measured in a gamma counter. The concentration of antigen is inversely proportional to the radioactivity measured in test tubes. By plotting binding values against a series of calibrators containing known amount of unconjugated Estriol, a calibration curve is constructed, from which the unknown concentration of unconjugated Estriol in patient samples can be determined.
This assay cannot measure the free (unbound) Estriol in serum. In fact this cannot be achieved by any commercial immunoassay method. If there is a need for measuring the free estriol, then the best method is the measurement of salivary estriol. Demeditec is offering such a testkit in ELISA format with the catalogue number DESLV3653.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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