CEA IRMA

产品名称：CEA IRMA
产    地：Demeditec 
产品货号：DE38100
产品规格：100 Tests
产品说明：
Special remarks:
1.Description
The I-125 CEA IRMA system provides a direct in vitro quantitative determination of human carcinoembryonic antigen (hCEA) in human serum in the range of 0-180 ng/ml. Each kit contains materials sufficient for 100 assay tubes permitting the construction of one standard curve and the assay of 41 unknowns in duplicate.
2.Introduction
Carcinoembryonic antigen (CEA) is a cell-surface glycoprotein with a molecular weight of 180-200kD, that occurs in high levels in colon epithelial cells during embryonic development. Levels of CEA are significantly lower in colon tissue of *****s, but can become elevated when inflammation or tumours arise in any endodermal tissue, including in the gastrointestinal tract, respiratory tract, pancreas and breast.
An overexpression of CEA protein has been detected in a variety of adenocarcinomas, including gastric, pancreatic, small intestine, colon, rectal, ovarian, breast, cervical and non-small-cell lung cancers. CEA is also expressed by epithelial cells in several non-malignant disorders, including diverticulitis, pancreatitis, inflammatory bowel disease, cirrhosis, hepatitis, bronchitis and renal failure and also in heavy smokers.
Therefore CEA should not be regarded as a tumour-specific marker for the screening of general population for undetected cancers. However, the determination of CEA levels provides important information about patient prognosis, recurrence of tumours after surgical removal and effectiveness of therapy.
3.Principle of the method
The technology uses two high affinity monoclonal antibodies in an immunoradiometric assay (IRMA) system.
The I-125 labelled signal-antibody binds to an epitope of the CEA molecule spatially different from that recognised by the biotin-capture-antibody. The two antibodies react simultaneously with the antigen present in standards or samples, which leads to the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”.
During a 2-hour incubation period with shaking the immuno-complex is immobilized to the reactive surface of streptavidin coated test tubes. Reaction mixture is then discarded, test tubes are washed exhaustively, and the radioactivity is measured in a gamma counter.
The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of calibrators containing known amount of hCEA, the unknown concentration of hCEA in patient samples can be determined.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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