PSA, free

产品名称：PSA free IRMA
产    地：Demeditec
产品货号：DE37100
产品规格：100 Tests
产品说明：
Special remarks:
Description
The 125I?free PSA IRMA system provides direct quantitative in vitro determination of human free Prostata Specific Antigen (fPSA) in human serum. Free PSA can be assayed in the range of 0?50 ng/ml using 100 µl serum samples.
Introduction
Prostate Specific Antigen (PSA) is a tissue-specific serine protease similar to the chymotrypsin- like glandular kallikreins. The active enzyme is a single chain glycoprotein of 237 amino acids (approximately 30 kDa). PSA is mainly responsible for gel dissolution in freshly ejaculated semen by proteolysis of the major gel forming proteins. The major part (70-90 %) of PSA in serum is complexed to alfa1-antichymotrypsin (ACT). Total PSA (free+ACT-complex) is increased in both benign prostate hyperplasia and malignant prostate cancer.
The prominent feature of fPSA immunoassays is their suitability for diagnostic and staging the prostate cancer. It has been shown that the free/total PSA ratio in benign hyperplasia is higher than in prostate cancer. According to the literature, the optimal free/total PSA cut-off value is 25%.
Principle of method
The technology uses two high affinity monoclonal antibodies in an immunoradiometric assay (IRMA) system.
The 125I labelled signal-antibody binds to an epitope of the fPSA molecule spatially different from that recognized by the biotin-capture-antibody. The two antibodies react simultaneously with the antigen present in standards or samples, which leads to the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”.
During a 2-hour incubation period with shaking immuno-complex is immobilized to the reactive surface of streptavidin coated test tubes. Reaction mixture is then discarded, test tubes washed exhaustively, and the radioactivity is measured in a gamma counter.
The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of calibrators containing known amount of fPSA, the unknown concentration of fPSA in patient samples can determined.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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