PTH

产品名称：PTH IRMA
产    地：Demeditec
产品货号：DE3649
产品规格：96 Tests
产品说明：
Special remarks:
Biological Activity
Human parathyroid hormone (hPTH) is a major physiological regulator of phosphocalcic metabolism. hPTH increases serum calcium concentrations by its actions on kidney (enhancing tubular Ca++ reabsorption and phosphate excretion) and bone (stimulating osteoclastic activity and bone resorption). It indirectly affects intestinal absorption of Ca++ by stimulating renal 1α-hydroxylation of 25 hydroxyvitamin D. The release of PTH is controlled in a negative feedback loop by the serum concentration of Ca++. PTH is synthesized in the chief cells of the parathyroid glands and secreted as an 84 amino acid molecule called "intact PTH", which is the main bioactive product. This molecule is degraded by proteolytic cleavage between amino acids 33-37 at peripheral sites to form biologically active amino-terminal fragments and biologically inactive carboxyl-terminal fragments. The carboxyl-terminal fragments are cleared only by glomerular filtration, while the bioactive intact PTH and amino-terminal fragments are also metabolically degraded in the liver and other tissues. The half-life of the carboxyl-terminal fragments increases dramatically in patients with renal failure. Thus, the measurement of intact PTH correlates best with the hormone production and biological activity.
Clinical Application
The measurement of intact hPTH by the present IRMA assay kit is used to establish the diagnosis of primary hyperparathyroidism by demonstrating elevated serum levels of bioactive PTH. It allows documenting the occurrence of secondary hyperparathyroidism in patients with Vit.D deficiency, intestinal malabsorption, or renal failure. In this last situation, the absence of interference with the inactive carboxyl-terminal fragments is especially valuable. The specificity and high sensitivity of the assay also allows differentiating clearly low serum PTH levels in hypoparathyroidism or in tumor-induced hypercalcaemia.
PRINCIPLES OF THE METHOD
The Demeditec hPTH-120 min-IRMA is a two-step immunoradiometric assay based on coated-tube separation. It allows the determination of intact human PTH (hPTH) in serum. Goat antibodies specific to the 1-34 hPTH fragment (N-terminal fragment) are attached to the lower and inner surface of the plastic tubes. Calibrators or samples are added to the tubes. After 1 hour incubation, washing removes the occasional excess of antigen, mid-regional and C-terminal fragments. 125I labelled monoclonal antibodies specific to the 44-68 hPTH fragment are added. After 1 hour incubation and washing the remaining radioactivity bound to the tube reflects the intact h-PTH concentration. This two-step IRMA is highly specific of the intact h-PTH and does not cross react with active and inactive fragments even at high concentrations as suggested by HACKENG et al.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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