Digoxin

产品名称：Digoxin ELISA
产    地：Demeditec 
产品货号：DE3268
产品规格：96 Tests
产品说明：
Special remarks:
SUMMARY AND EXPLANATION OF THE TEST
The clinical usefulness of the measurement of serum digoxin (DIG) is due to its low therapeutic ratio; a very small difference exists between therapeutic and toxic tissue levels.  In addition, individuals may vary in their response to digoxin with an apparent increase in susceptibility to toxicity with age. The action of digoxin is to increase the force and velocity of myocardial contraction.   This is necessary in the treatment of congestive heart failure and arrhythmias such as atrial fibrillation and atrial flutter. The myocardial concentrations of digoxin to serum levels remain relatively constant during normal renal function.  This distribution ratio of digoxin is approximately 29 to 1 between the heart and serum.  Thus, monitoring digoxin therapy by measurement of serum levels is feasible from the pharmacological standpoint, since serum levels are related to tissue levels following post-absorption equilibration.  A practical and sensitive method of digoxin quantitation in serum is by enzyme immunoassay.
This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations.  In this method, serum reference, patient specimen, or control is first added to a microplate well.  Digoxin coupled with enzyme horseradish peroxidase (HRP) is added, and the reactants are mixed. A competition reaction results between the enzyme conjugated digoxin and the native digoxin for a limited number of antibody combining sites immobilized on the well.
After the completion of the required incubation period, the antibody bound enzyme-digoxin conjugate is separated from the unbound enzyme-digoxin conjugate by aspiration or decantation.  Excess unbound enzyme reagent is washed via a wash step. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color.
The employment of several serum references of known digoxin concentration permits construction of a graph of activity and concentration.  From comparison to the dose response curve, an unknown specimen's activity can be correlated with digoxin concentration. The concentrations are expressed in ng/ml.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

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