Cortisol in Urine

产品名称：Urinary Cortisol ELISA
产    地：Demeditec 
产品货号：DE2989
产品规格：96 Tests
产品说明：
Special remarks:
1 INTENDED USE
Competitive immunoenzymatic colorimetric method for quantitative determination of Free Cortisol concentration in Urine. Urinary “Free” Cortisol kit is intended for laboratory use only.
1.1 CLINICAL SIGNIFICANCE
Cortisol is a steroid hormone released from the adrenal cortex in response to an hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid-binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors. These normal endogenous functions are the basis for the physiological consequences of chronic stress - prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs. The free cortisol fraction represents the metabolically active cortisol. In normal conditions, less then 1% it comes excrete in urines. In pathological conditions (syndrome of Cushing) the levels of free urinary cortisol are elevate, because the CBG don’t bound the plasmatic cortisol in excess and it was remove with urines. During pregnancy or estro-progestogen treatment an increase of plasmatic cortisol caused by an increment of the production of the transport protein, but the levels of free urinary cortisol results normal to indicate a correct surrenic functionality. This test is very useful to estimate the real surrenic function, because is dose the free cortisol, it is the metabolically active form. Moreover the measurement of free urinary cortisol is the better parameter for the diagnosis of the Cushing’s syndrome.
2 PRINCIPLE
The Cortisol (antigen) in the sample competes with the antigenic Cortisol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Cortisol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (acidic solution) is added. The colour intensity is inversely proportional to the Cortisol concentration of in the sample. Cortisol concentration in the sample is calculated through a calibration curve.
The above test kits measures the conjugated Cortisol in urine. Historically this has been a useful but only an indirect measurement for the free Cortisol in plasma or serum. Meanwhile there is also a direct method available for the measurement of free Cortisol. This has to be done in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DES6611.
上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于国内**地位，公司提供的产品涵盖了二十多个国家近五十万种产品，而且产品的数量与信息在不断的增长和更新，公司提供的产品能够使生命科学工作者加快对生物化学，分子生物学，细胞生物学以及蛋白质组学研究的认知，以及分子诊断和临床医学领域的应用。

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!