Rabbit TrueBlot Set including IP Beads **

产品名称：Rabbit TrueBlot™ Set including IP Beads 50 ul (1000X)
产品货号：eBioscience 88-1688-31
产品规格：50 uL
Rabbit TrueBlot® Set (with IP beads)
RUO: For Research Use Only. Not for use in diagnostic procedures.
SKU# 88-1688
Cat. No. Size
88-1688-31  50 uL 
Description: Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/WB data with Rabbit IgG TrueBlot, simply substitute the conventional HRP anti-rabbit IgG blotting reagent with Rabbit IgG TrueBlot™ and follow the prescribed protocol for sample preparation and immunoblotting.
Note that there are three key procedural considerations:
1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-mouse IgG beads, anti-rat IgG beads, or anti-rabbit IgG beads should be used for mouse, rat, or rabbit immunoprecipitating antibodies, respectively.
2. Immunoprecipitate should be completely reduced.
3. Milk should be used as the blocking protein for the immunoblot.

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