Phospho-AKT 1/2/3 (Ser473) InstantOne ELISA **

产品名称：Phospho-AKT 1/2/3 (Ser473) InstantOne ELISA
产品货号：eBioscience 85-86042-11
产品规格：96 tests
phospho-AKT 1/2/3 (Ser473) InstantOne™ ELISA
Also known as: PKB, Protein Kinase B, RAC-PK-alpha
RUO: For Research Use Only. Not for use in diagnostic procedures.
Attention: Please contact Technical Service for a lot specific COA
SKU# 85-86042
Cat. No. Size
85-86042-11  96 tests 
85-86042-103  10 x 384 tests 
85-86042-253  25 x 384 tests 
85-86042-503  50 x 384 tests 
Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 in cell lysates. Detection of rat AKT 1/2/3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.

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