Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI,胰朊酶抑制剂, 胰蛋

Synonym:

SBTI

CAS Number:

9035-81-8

EC Number:

232-906-9

MDL number:

MFCD00082103

Analysis Note

Protein determined by biuret.

One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

Extremely stable: a 1% sterile filtered solution maintained activity for over 3 years when kept refrigerated.

View more information on Trypsin Inhibitor.

Preparation Note

Chromatographically prepared

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Unit Definition

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

One chymotrypsin unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Biochem/physiol Actions

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x10⁸.

type

Type I-S

form

lyophilized powder

mol wt

α-chain mol wt 20090 Da by calculation

β-chain mol wt 20036 Da by calculation

γ chain mol wt 20163 Da by calculation

EQP level

Premium

solubility

H₂O: 10 mg/mL

phosphate buffer: 10 mg/mL (hazy above 10 mg/ml)

storage temp.

2-8°C

Personal Protective Equipment

Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

WGK Germany

3

品名

产地

货号

规格

单价

备注

Trypsin Inhibitor 胰蛋白酶抑制剂

Sigma

T9003

25mg

询价

现货

Trypsin Inhibitor 胰蛋白酶抑制剂

Sigma

T9003

100mg

询价

现货