地高辛标记检测试剂盒II,DIG-High Prime DNA Labeling and Detection Starter Kit II

地高辛标记检测试剂盒II

比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。-20℃保存。

·          随机引物法标记探针

·          AP标记抗体

·          底物NBT/BCIP

·          化学发光法检测

Roche相关资料:

产品英文名称:DIG-High Prime DNA Labeling and Detection Starter Kit II

Cat. No. : 11585614910

Pack size: 1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm²

Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1. DIG-High Prime, 5x conc., 50 µl
2. DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
3. DNA Dilution Buffer, 3 x 1 ml
4. Anti-digoxigenin-AP Conjugate, 50 µl
5. CSPD, ready-to-use, 50 ml
6. Blocking Solution, 10x conc., 4 x 100 ml
7. DIG Easy Hyb Granules, 4 x 100 ml

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