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merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtr

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产品名称: merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtr
产品型号: merck 539790-1KIT
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merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtract 亚细胞蛋白质组抽


merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtr  的详细介绍
merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtract 亚细胞蛋白质组抽

ProteoExtract 亚细胞蛋白质组抽提试剂盒

 

MERCK相关产品资料：

539790  ProteoExtract Subcellular Proteome Extraction Kit ( ProteoExtract® Subcellular Proteome Extraction Kit )

S-PEK Kit

Fast and reproducible extraction of subcellular proteomes from mammalian cells

ProteoExtract Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells. The S-PEK takes advantage of the different solubilities of certain subcellular compartments in the four selected reagents. In the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. Cells or the parts of the cells remain attached to the plate during sequential extraction of subcellular compartments, until the appropriate extraction reagent is used. Thus, the early destruction of the cellular structure by enzymatic or mechanical detachment of cells from the tissue culture plate and any mixing of different subcellular compartments is prevented. For suspension-grown cells, extraction starts with gentle sedimentation and washing of the cells. The stepwise extraction delivers four distinct protein fractions from one sample:

  • Cytosolic fraction (F1)
  • Membrane/organelle protein fraction (F2)
  • Nucleic protein fraction (F3)
  • Cytoskeletal fraction (F4)

Proteins are obtained in the native state making the S-PEK suitable for many downstream applications such as 1D and 2D gel electrophoresis, immunoblotting, enzyme activity assays, and protein microarrays.

Sample size: 3-5x106 or 25-50 mg tissue.

Product number

型号

价格

备注

539790-1KIT

1 kit

询价

 

 

产品信息

Form

20 Extractions

Avoid freeze/thaw

Yes

Sample type

Mammalian tissue, cultured mammalian cells

Kit contains

Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.

Features and benefits

  • Stepwise extraction resulting in four distinct subcellular proteomes from one sample
  • Highly reproducible
  • No ultracentrifugation steps
  • Fast—needs just 2 hours with 45 minutes hands-on time
  • Produces proteins suitable for functional studies

 

Store and ship information

 

Storage

+2°C to +8°C

Ship

Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS

 

**标示

S Phrase

S: 22-26-28.2-36/37-45

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
After contact with skin, wash immediately with soap and plenty of water.

In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

R Phrase

R: 22-36/38-52/53

Harmful if swallowed.

 

 

 

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